1. 染料试剂

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied。

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample。 The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima。

Dye Reagents (253):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0940
    H2DCFDA 4091-99-0 99.82%
    H2DCFDA是可渗透细胞,用于检测细胞内活性氧 (ROS) 的探针。
    H2DCFDA
  • HY-15534
    JC-1 3520-43-2 99.46%
    JC-1是荧光亲脂性羰花青染料,用于测量线粒体膜电位。
    JC-1
  • HY-12591A
    D-Luciferin 2591-17-5 98.81%
    D-luciferin 是萤光素酶的天然底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin
  • HY-D0814
    DAPI dihydrochloride 28718-90-3 >98.0%
    DAPI二盐酸盐(4',6-二脒基-2-苯基吲哚)是结合富含A-T碱基对DNA序列的荧光染色。
    DAPI dihydrochloride
  • HY-D0938
    CFSE 150347-59-4
    CFSE 是可以跟踪细胞分裂的荧光染料。
    CFSE
  • HY-D0718
    Nile Red 7385-67-3
    Nile Red是在疏水环境下的强荧光染料,用于检测细胞内脂滴。
    Nile Red
  • HY-D0815
    Propidium Iodide 25535-16-4 >98.0%
    Propidium iodide是可用于细胞染色的红色荧光染料。
    Propidium Iodide
  • HY-D0913
    1-Methyl-7-nitroisatoic anhydride 73043-80-8 >98.0%
    1-methyl-7-nitroisatoic anhydride是一种检测局部核苷酸的试剂。
    1-Methyl-7-nitroisatoic anhydride
  • HY-66019
    FITC 3326-32-7 >98.0%
    FITC是荧光素的衍生物,用于标记伯胺。
    FITC
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.87%
    Hoechst 33342 trihydrochloride是可以膜透细胞膜,发蓝色荧光的 DNA 染料。
    Hoechst 33342 trihydrochloride
  • HY-D0041
    Calcein-AM 148504-34-1 >98.0%
    Calcein-AM是用于测定细胞活力的可以渗透细胞的荧光染料。
    Calcein-AM
  • HY-12591B
    D-Luciferin potassium salt 115144-35-9 99.20%
    D-Luciferin potassium salt 是萤光素酶的底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin potassium salt
  • HY-111382
    Diphenylterazine 344940-63-2
    Diphenylterazine 是一种生物发光剂。
    Diphenylterazine
  • HY-D0711
    Cardiogreen 3599-32-4 >98.0%
    Cardiogreen是一种低毒性荧光剂,广泛用于医学诊断,如确定心输出量,肝功能和肝血流量,以及用于眼科血管造影。
    Cardiogreen
  • HY-D0083
    DiI 41085-99-8 99.62%
    DiI(DiIC18(3))是亲脂性膜染料,侧向扩散染色整个细胞。 DiI被激发后发射橙红色荧光。 荧光在水中很微弱,但是结合到细胞膜中时荧光信号强且具有光稳定性。
    DiI
  • HY-D0079
    Dihydroethidium 104821-25-2 99.77%
    Dihydroethidium(Hydroethidine; PD-MY 003)是过氧化物指示剂,在细胞质中具有蓝色荧光,直到被氧化。
    Dihydroethidium
  • HY-D0218
    Thioflavin T 2390-54-7 98.09%
    Thioflavin T 是一种阳离子 Benzothiazole 染料,在组织切片中与淀粉样蛋白结合后荧光强度增强。
    Thioflavin T
  • HY-D0098
    Fluorescein-5-maleimide 75350-46-8 98.00%
    Fluorescein-5-maleimide 是一种硫醇反应荧光染料,可用于将荧光素与蛋白质缀合(激发波长:494nm,发射波长:519nm)。
    Fluorescein-5-maleimide
  • HY-15928
    PNPP 4264-83-9 99.85%
    PNPP 是用于 ELISA 和常规分光光度测定的碱性和酸性磷酸酶的非蛋白质性显色底物。
    PNPP
  • HY-15925
    NBT 298-83-9 >99.0%
    NBT(Nitro BT;p-Nitrotetrazolium blue)是脱氢酶底物,与碱性磷酸酶底物BCIP一起用于western blotting和免疫组化染色。
    NBT
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